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Allelic variation at the Vrn-A1, Vrn-B1, Vrn-D1, Vrn-B3 and Ppd-D1a loci of Pakistani spring wheat cultivars Electron. J. Biotechnol.
Iqbal,Muhammad; Shahzad,Armghan; Ahmed,Iftikhar.
Flowering time in bread wheat (Triticum aestivum L.) is controlled by vernalization and photoperiod response, and earliness per se genes. The genetic basis of flowering time has not been investigated in Pakistani bread wheat. This study was, therefore, conducted to determine the allelic composition at Vrn-A1, Vrn-B1, Vrn-D1, Vrn-B3 and Ppd-D1a loci of 59 Pakistani spring bread wheat cultivars. These cultivars, along with 4 isogenic lines for vernalization genes were characterized with previously reported DNA markers designed for detecting allelic variation at 4 Vrn (Vernalization) and 1 Ppd (Photoperiod) loci. Spring habit Vrn-A1a allele was found in 36% cultivars either alone or with spring habit Vrn-B1 and Vrn-D1 alleles. Two wheat cultivars had the...
Tipo: Journal article Palavras-chave: Photoperiod; Spring growth habit; Triticum aestivum; Vernalization.
Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000100001
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Assessment of genetic diversity among Pakistani wheat (Triticum aestivum L.) advanced breeding lines using RAPD and SDS-PAGE Electron. J. Biotechnol.
Ahmed,Muhammad Farooq; Iqbal,Muhammad; Masood,Muhammad Shahid; Rabbani,Malik Ashiq; Munir,Muhammad.
Genetic diversity was assessed among 32 advanced wheat breeding lines included in the National Uniform Wheat Yield Trials (2006-07) of Pakistan using molecular (DNA) and biochemical (SDS-PAGE) markers. Of the 72 RAPD primers used for initial screening, 15 were found polymorphic. A total of 140 bands (61.4% polymorphic) were generated by the 15 random decamer primers. Genetic similarity coefficients ranged from 0.81 to 0.94 for rainfed and from 0.70 to 0.93 for the normal seeding date group. Cluster analysis using the unweighted pair group method of arithmetic averages (UPGMA) clustered the 32 advanced wheat breeding lines into one major and three small groups. Maximum level of polymorphism (90%) was observed for the primer OPA-05. Lines N9 and N11 showed...
Tipo: Journal article Palavras-chave: Genetic diversity; Molecular markers; Polymorphism; RAPD; SDS-PAGE; Wheat.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000300001
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Evaluation of different detection methods of biofilm formation in the clinical isolates BJID
Hassan,Afreenish; Usman,Javaid; Kaleem,Fatima; Omair,Maria; Khalid,Ali; Iqbal,Muhammad.
BACKGROUND: Microorganisms growing in a biofilm are associated with chronic and recurrent human infections and are highly resistant to antimicrobial agents. There are various methods to detect biofilm production like Tissue Culture Plate (TCP), Tube method (TM), Congo Red Agar method (CRA), bioluminescent assay, piezoelectric sensors, and fluorescent microscopic examination. OBJECTIVE: This study was conducted to compare three methods for the detection of biofilms. METHOD: The study was carried out at the Department of Microbiology, Army Medical College, National University of Sciences and Technology, Pakistan, from January 2010 to June 2010. A total of 110 clinical isolates were subjected to biofilm detection methods. Isolates were identified by standard...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biofilms; Bacteria; Anti-bacterial agents.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000400002
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Salt tolerance and regulation of gas exchange and hormonal homeostasis by auxin-priming in wheat PAB
Iqbal,Muhammad; Ashraf,Muhammad.
The objective of this work was to assess the regulatory effects of auxin-priming on gas exchange and hormonal homeostasis in spring wheat subjected to saline conditions. Seeds of MH-97 (salt-intolerant) and Inqlab-91 (salt-tolerant) cultivars were subjected to 11 priming treatments (three hormones x three concentrations + two controls) and evaluated under saline (15 dS m-1) and nonsaline (2.84 dS m-1) conditions. The priming treatments consisted of: 5.71, 8.56, and 11.42 × 10-4 mol L-1 indoleacetic acid; 4.92, 7.38, and 9.84 × 10-4 mol L-1 indolebutyric acid; 4.89, 7.34, and 9.79 × 10-4 mol L-1 tryptophan; and a control with hydroprimed seeds. A negative control with nonprimed seeds was also evaluated. All priming agents diminished the effects of salinity...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Triticum aestivum; Hormonal balance; Plant growth regulators; Salinity; Seed priming; Stomatal regulation.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2013000900004
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